Glycerol inhibition of proton-dependent binding of ligands to haemoproteins [proceedings].

Manchester, 1970), this electrical stimulation did not affect protein synthesis in vitro, although effects have been observed in isolated rat hemi-diaphragm (Reeds et al., 1979). However, a similar increase in tension produced by intermittent stretching (mechanical stimulation: lOOms at 3 s intervals) was associated with increased synthesis of total protein and collagen (Table 1). Where measured, the rate of actomyosin synthesis was similar to that of total protein and was increased in the same proportion by mechanical stimulation. No differences in total protein synthesis were observed between muscles held at fixed tensions of 2g or l o g for 6h. It is unlikely that a change in the supply of amino acids provides an explanation for these effects, as mechanical stimulation changed neither the rate of uptake of proline nor the intracellular concentration or equilibrium specific radioactivity. It is possible that mechanical stimulation increases the amount of energy supplied to the muscle by increasing the rate of uptake of glucose from the medium and the penetration of oxygen throughout the tissue.